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Do multiple thread request share same singleton beans in Spring?

I have been trying to understand spring beans. As per my understanding, by default all beans are singleton and a singleton bean with lazy-init property set to true is created when it is first requested and singleton bean with lazy-init property set to false is created when the application context is created.
So, in an application, when a user request comes in ( where each request is a separate thread), do all these threads share the same singleton beans when requested within the program/class?

Yes, if the bean is created with default scope, the bean is shared across threads. However, another scope can be used to achieve the behaviour you mentioned.
See: https://docs.spring.io/spring-framework/docs/3.0.0.M3/reference/html/ch04s04.html?

Yes, by default (scope == 'singleton'), all threads will share the same singleton bean. There are two other bean scopes, session and request, that may be what you're looking for. The request scope creates a bean instance for a single HTTP request while session scope maintains a unique bean for each HTTP Session.
For a list and description of all of the Spring bean scopes, check out: Spring bean scopes

The best way to understand this is to understand how #Autowired annotation works.
Or in other words to understand "Spring Dependency Injection".
You said, "
by default all beans are singleton
We use #Autowired when injecting one layer into another between two layers.
If exemplify: Suppose, I want to inject UserService UserController. UserService is a Singleton bean. Creates an instance from UserService and Stores its cache. When we want to inject this service with #Autowired annotation. This annotation brings the UserService stored in the cache.
In this way, Even if we do this inject operation in many classes.#Autowired inject an instance of UserService with singleton bean instead of dealing with one UserService at each time. It saves us a big burden.
This is the fundamental working logic of Spring Singleton Bean.
Also, Spring Ioc Container manages this whole process.

Call init-method of a session-scoped bean from a singleton bean

I have a session scoped bean which is injected into a singleton bean using a proxy.
From the singleton bean I need to call a certain init-method of the session scoped bean, which needs the singleton bean as parameter.
Furthermore I can not change the source of the session scoped bean.
To elaborate the case:
The singleton bean is a Service and the session scoped bean is a Vaadin4Spring eventbus, for which I need to call the method eventBus.subscribe(this).
There seems to be no way to check, if I am already subscribed and I must not call subscribe twice on a certain eventBus instance.
Is there a way to tell Spring to call eventBus.subscribe(this) when the proxy links to an eventBus instance for the first time?

Can't you just go the other way round, inject the service into your eventbus, and call this.subscribe(serviceBean) in a #PostConstruct annotated method in the eventbus? Then it would only be called once when the eventbus is constructed.

You could add #Lazy to the Singleton. You do not need the Proxy.

Inject HttpServletRequest into Controller

As I know per default are controllers in Spring MVC singletons. HttpServletRequest passed offen to the controller handler method. And its ok, while HttpServletRequest is request-scoped, but I see often HttpServletRequest gets #Autowired into the controller field, like this:
#Controller("CMSProductComponentController")
#RequestMapping(CMSProductComponentController.CONTROLLER_PATH)
public class CMSProductComponentController {
#Autowired
private HttpServletRequest request;
}
Could be this a problem? And more general question: What happens if inject a reqeust-scoped component into a singleton?

No, for HttpServletRequest it will not be a problem and it shouldn't for other request scoped beans. Basically, Spring will generate a proxy HttpServletRequest that wraps some kind of ObjectFactory (RequestObjectFactory for HttpServletRequest) (YMMV) that knows how to retrieve the actual instance. When you use any of the methods of this proxy, they will delegate to that instance.
What's more, this is done lazily, so it won't fail at initialization. It will however fail if you try to use the bean when there is no request available (or if you haven't registered the RequestScope).
The following is in response to the comments and to clarify in general.
Regarding the proxy-mode attribute of #Scope or the XML equivalent, the default is ScopedProxyMode.NO. However, as the javadoc states
This proxy-mode is not typically useful when used with a non-singleton
scoped instance, which should favor the use of the INTERFACES or
TARGET_CLASS proxy-modes instead if it is to be used as a dependency.
With request scoped beans, this proxy-mode value will not work. You'll need to use INTERFACES OR TARGET_CLASS depending on the configuration you want.
With scope set to request (use the constant WebApplicationContext.SCOPE_REQUEST), Spring will use RequestScope which
Relies on a thread-bound RequestAttributes instance, which can be
exported through RequestContextListener, RequestContextFilter or
DispatcherServlet.
Let's take this simple example
#Component
#Scope(proxyMode = ScopedProxyMode.INTERFACES, value = WebApplicationContext.SCOPE_REQUEST)
public class RequestScopedBean {
public void method() {}
}
...
#Autowired
private RequestScopedBean bean;
Spring will generate two bean definitions: one for your injected bean, a singleton, and one for the request scoped bean to be generated on each request.
From those bean definitions, Spring will initialize the singleton as a proxy with the types of your target class. In this example, that is RequestScopedBean. The proxy will contain the state it needs to produce or return the actual bean when it is needed, ie. when a method is called on the proxy. For example, when
bean.method();
is called.
This state is basically a reference to the underlying BeanFactory and the name of the request-scoped bean definition. It will use these two to generate a new bean and then call method() on that instance.
The documentation states
The Spring IoC container manages not only the instantiation of your
objects (beans), but also the wiring up of collaborators (or
dependencies). If you want to inject (for example) an HTTP request
scoped bean into another bean, you must inject an AOP proxy in place
of the scoped bean. That is, you need to inject a proxy object that
exposes the same public interface as the scoped object but that can
also retrieve the real, target object from the relevant scope (for
example, an HTTP request) and delegate method calls onto the real
object.
All eagerly loaded request scoped beans, if implemented correctly, will be proxies. Similarly, request scoped beans that aren't eagerly loaded will either be proxies themselves or be loaded through a proxy. This will fail if there is no HttpSerlvetRequest bound to the current thread. Basically, a proxy is necessary somewhere in the bean dependency chain for request scoped beans.

What happens if inject a reqeust-scoped component into a singleton?
Try it and you'll get a BeanCreationException¹ during application context initialization. The error message clearly explains why this doesn't happen with HttpServletRequest:
Scope 'request' is not active for the current thread; consider defining a scoped proxy for this bean if you intend to refer to it from a singleton;
So obviously HttpServletRequest is a scoped proxy. If you want to use beans of smaller scopes in singletons they have to be proxies. The documentation elaborates about smaller scoped dependencies in Scoped beans as dependencies.
[1]: unless you didn't change the default behaviour for proxyMode, which is NO or try to inject it with #Lazy. The latter might result into a valid application context but might lead to request scoped beans acting like singletons (e.g. if a request scoped bean is injected into a singleton).

Spring beans and changing bean parameter in application life cycle

I have a web application that is based on Spring. There is defined a bean that holds some class MyClass that is passed also with beans to MyEndpoint that extends AbstractMarshallingPayloadEndpoint.
MyClass has set some boolean parameter in beans to true.
If my application will change this parameter programically to false, does next request will have it also set to false or it will contains a default bean parameter - true ?

It depends on the scope of the bean (which will default to singleton, if you don't specify one).
If it is of singleton scope, there is one instance of that bean in the application context, and each time you ask for that bean, you get that single instance. If you change it in a request with this scope, then the change will be maintained.
If it is of prototype scope, a new instance is given to you (created with the same parameters) each time you ask the application context for it. If you change it in a request with this scope, then the change will be ignored when you get another instance of this object.
These are the two most commonly used (at least with my time in Spring). There are other scopes (request, session, globalsession), but you should read the documentation on them that Spring provides.

If you want this property to change dynamically according to your application business logic and not only to be reset on every new request (otherwise Request scope will do the magic) consider Factory Methods (especially Lookup Method Injection)

Spring #Autowired and WebApplicationContext in Tomcat

#Autowired works only once.
What to do to make it wire the bean every time the Servlet is recreated?
My web-app (Tomcat6 container) consists of 2 Servlets. Every servlet has private fields.
Their setters are marked with #Autowired
In the init method I use
WebApplicationContextUtils
...
autowireBean(this);
It autowires the properties marked with #Autowired once - during the initialization of the Servlet.
Any other session will see these fields values, they will not be rewired after the previous session is destroyed.
What to do to make them rewire them each time a Servlet constructor is called?
a) Put the autowiring into the constructor?
Or better 2) get a web app context and extract a bean from there?

There seems to be some misunderstanding about how the container works. Servlets are essentially singletons, you don't get a new servlet everytime someone calls the server. Storing state in private fields on a servlet is pretty much an error.
What is the scope and life-cycle of the stateful part of your request processing? If it's just the life of the request then you can take whatever on your servlet is stateful and move it into another class. Then you can define a prototype bean for that class and use getBean at the start of the request to get a new one. If you want to start getting fancy you could write a filter that puts a new bean into a ThreadLocal at the start of each request.
If your state needs to span multiple requests, you need to start keeping state or a key that points to the state storage on the web session, or look into using a conversation framework.

Try using scope as prototype for that bean #Scope("prototype")

You may try to use #Scope("session")